Introduction to Laser Beam & Spectral Measurement - CVI Melles Griot 2009 Technical Guide, Vol 2, Issue 1
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SUPLEMENTAL DATA 1 (S1) : Addendum to Experimental Methods
A 488 nm argon laser (Melles Griot) was used as the excitation source and the laser beam was focused using a 100X, 1.3 N.A., oil immersion apochromatic objective (Olympus). Throughout experiments excitation laser power was kept at 150 μW using neutral density filters. The emitted fluorescence was then collected by the same objective and an initial dichroic mirror (505DCXE, Chroma Technology) se...
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